6Pcs Wide Mouth Mushroom Liquid Culture Lid Grain Spawn Cultivation Mason Jar Lid with 0.2 micron PTFE Syringe Filter & 20mm Self Healing Injection Ports For Mushroom Growing Mycology(Golden)

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I think dispensing a drop or two right after flame then immediately into the injection port is my only option. Our organic rye is nutrient-enriched with gypsum and other PH adjusters to give you a fast colonizing jar from spores, agar or liquid culture. I like to pre-cook my grains before sterilization so that they can soak up water, then steam dry them so that they completely dry off on the outside. com, the Sell on Etsy app, and the Etsy app, as well as the electricity that powers Etsy’s global offices and employees working remotely from home in the US. I’ve never done this but as long as the fundamental principles of sterile working are followed, I can’t see a reason that it wouldn’t work!

There is no need to “incubate” grain at warm temperatures in the dark, since the mycelium will grow happily in normal room temperature conditions. Another option is to drill a few holes in the lid and place a synthetic filter disc between the jar lid and the screw cap. Place your scalpel in the flame of the butane torch; when the tip glows red, it has been sterilized. Glad you like the site 🙂 You can usually do 2-3 grain to grain expansions before running into issues- If I were you, I would start with LC to grain in mason jars, then expand that 10X into 5 lb bags.

Punch your holes in the same place on the lid, making sure the outer-most hole isn’t too close to the edge of the lid as the syringe filter will overhang some-what. To enable personalised advertising (like interest-based ads), we may share your data with our marketing and advertising partners using cookies and other technologies. If you are propagating plants, we recommend taking out your plant cuttings after about a month so the roots don't get stuck inside. Then fold it back on itself and back on itself again, do this until it is a thick web of polyfill but not overly ‘tight’. Please be sure to read your pressure canner’s instructions and familiarize yourself with its safety features BEFORE trying this step.

If you were to use a Syringe filter to inoculate, you would likely filter out much of the mycelium and block up the port but also introduce a good avenue for contamination to ‘wick’ through if that makes sense. If it is possible, then it means that the difference between grain to grain method to make spawn and growing mushroom colonizing substrate is in the usage of closed jars that don’t allow fruiting as apposed to the bags with the holes?We will process the personal data you have supplied to communicate with you in accordance with our Privacy Policy. Once used as an actual filter, it’s gas exchange ability would be reduced severely as it would be gunked up with mycelium. Take your syringe filter and on the barbed side (that would fit into a luer lock syringe) put a bead of epoxy on the blue rim of it.