According to a study by Ozemek et al., healthy diets like DASH dietary patterns have been shown to decrease SBP by 11 mm Hg in hypertensive individuals and by 3 mm Hg in normotensive individuals. Ideally, the goal is to reduce sodium to <1500 mg/dl, but it is good to aim for at least a 1000 mg/day reduction, which has been shown to reduce SBP approximately by 5/6 mm Hg in hypertensive individuals and by 2/3 mm Hg in normotensive individuals [ 11]. Furthermore, Ozemek et al. revealed in their study that increasing dietary potassium intake with a goal of 3500-5000 mg/day has been shown to reduce SBP by −4/5 mm Hg in hypertensive individuals and by −2 mm Hg in normotensive individuals [ 11]. The upholstery suffers most. We have HG car upholstery cleaner. Or take a look at our tips for cleaning fabric car upholstery. For leather seats, we have HG deep cleaner for leather. To inspect the consequence of Hg 2+ concentration on the morphology, the bacterial cells were freshly grown in the presence (50 and 100 mg/L - test samples) and absence (control) of Hg 2+ as HgCl 2; and cell pellets were harvested by centrifugation (5000 rpm at 4°C for 5 min) after 48 h. SEM analysis has been carried out, as reported by Joshi etal. (2021). In brief, the bacterial cells were fixed with 2.5% glutaraldehyde followed by the post-fixing in 1% osmium tetroxide and dehydration of the cells with graded ethanol series (25%, 50%, 80%, 90%, and absolute). The processed samples were scanned using SEM (JEOL-JSM-IT500). Extraction of metabolites and Gas Chromatography-Mass Spectrometry (GC-MS) analysis The modification in functional groups present in the culture pellets was identified by measuring the spectra in the range of 400 to 4000 cm -1 using Fourier transform infrared (FT-IR) spectroscopy (IR Affinity-I spectrometer, Shimadzu, Japan), as described by Joshi etal. (2021). In brief, 48 h grown cultures in ZMB medium without Hg supplementation were used as a control, whereas cultures with 50 mg/L of Hg supplementation were used as Hg treated. The mixtures (lyophilized cells and 2% KBr) were fixed in the FT-IR spectrometer after compressing them into translucent sample discs, followed by analyzing in ATR-FT-IR mode by following the manufacturer’s protocol. Scanning Electron Microscopy (SEM) analysis of MRB

The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/ Supplementary Material. Author contributions Our preliminary study suggests that the detoxification of Hg 2+ is the immediate result of the GST and merA function as shown in Figure S6. The existence of the glutathione reductase gene in some bacterial mer operons also supports the role of LMW thiols in Hg 2+ detoxification ( Norambuena etal., 2018). The E. coli, together with the integrated merA - GST gene, was able to survive in the high Hg stress environment and transform Hg 2+ to Hg 0 ( Cursino etal., 2000). It is correlated that IOTG may act as Hg(II)-buffering agents and subsequently, Hg 2+ is reduced by merA. In the case of merA, mercuric ion was uptaken by the active process i.e. mer mediated transport (merP and merT) and converted to Hg 0 form by mercuric reductase. On the other hand, the Hg 2+ that was uptaken through the passive process could subsequently be inactivated by GST or conjugated to merA. Thus, an alternative method is proposed via which Hg resistance level may be augmented in bacteria: the sequestering of Hg given a protein-ligand interaction may lead to an improved way for the volatilization process. ConclusionsMany studies suggest that mer operon is located on any one of either location such as mobile elements like plasmids, transposons, genomic or chromosomal DNA ( Liebert etal., 1999; Schelert etal., 2004; Zeng etal., 2010). The mer operon contains many functional genes along with the operator, promoter, and regulatory elements. All functional genes encrypt for the diverse proteins that contribute to the detection, transportation, and reduction of Hg ions ( Barkay etal., 2003; Dash and Das, 2012; Naguib etal., 2018). An extremely noteworthy positive correlation was found among the harboring of mer gene with the phenotypic resistance to Hg and concentration of the Hg in the source environment ( Osborn etal., 1997). In this study, we did not find any Hg from the ecosystem (detectable limit - 0.73 µg/L), but the amplification of mer genes and available literature suggested that these are extensively dispersed in a non-highly contaminated or contamination-free environment such as an open ocean, Antarctic sea-ice, high Arctic snow, sea ice brine etc. and plays a key role in the biogeochemical cycle of Hg ( Christakis etal., 2021). Thus, the Hg adaptation by these isolates is reasonably accepted. Confirmation of Hg 2+ reduction by the isolates Increased physical activity has been advocated as the first-line intervention for preventing and treating patients with prehypertension and as a treatment strategy for patients with stage 1 or stage 2 HTN, according to the Duthe American College of Sports Medicine, the United States Joint Nations Committee on Prevention, Detection, Evaluation, and Treatment of High BP, the World Health Organization and International Society of Hypertension, and The National Heart Foundation [ 36]. Exercise can consequently help prevent prehypertension from progressing and can help reduce or stop medications prescribed for the treatment of stage 1 HTN [ 37]. Mercury (Hg) pollution poses a global threat to human and environmental health due to its noxiousness, mobility, and lengthy residence duration in the atmosphere ( Raphael etal., 2011). Toxic metals rapidly accumulate in the food chain, impacting higher trophic levels, hence these are of principal concern nowadays ( Raphael etal., 2011). According to recent research, oceanic release and biomass burning (organic compounds) account for the majority of worldwide Hg emissions, with anthropogenic activities accounting for the remaining significant percentage ( Pirrone etal., 2010; Nelson etal., 2012; Serrano etal., 2013). Hg exists in elemental, inorganic, and organic forms in both land and water systems, depending on oxidation-reduction conditions. In the atmosphere, Hg with valence +2 is more extensively spread ( Wang etal., 2004). In the biogeochemical cycle, a considerable part of Hg accumulates in seas and oceans by its atmospheric deposition ( Bindler, 2003; Wang etal., 2004). The effects of human-induced sources on the Hg contents and its forms are extensively larger in the photic layers of oceans ( Strode etal., 2007).

Centre for Ocean Science and Technology for Islands, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Port Blair, India

Shine restoring car dashboard cleaner

Three experimental replicates were determined for each analysis and the statistical significance of the results was analyzed by one way ANOVA test. All Statistical analyses were carried out using SPSS (version 17) and GraphPad Prism 7 software. Differences were considered significant at p< 0.05 and values are reported as mean ± standard deviation (SD). Results and discussion According to the American College of Cardiology, a BP higher than 180/120 mm Hg is considered a hypertensive emergency or crisis. Patients with these blood pressures need emergency medical help. Untreated high BP may increase the risk of myocardial infarction, stroke, and other serious complications. Monitoring BP every two years, starting at age 18, is important to diagnose and treat hypertension timely to prevent complications. HTN is diagnosed by performing repeated careful measurements of blood pressure. Blood pressure is categorized as follows: Normal blood pressure, defined as systolic blood pressure (SBP) less than 120, and diastolic blood pressure (DBP) less than 80. An elevated BP is an SBP of 120 to 129 and a DBP of less than 80. HTN is defined as a systolic pressure more than or equal to 130 or a diastolic pressure more than or equal to 80.

GJ: Conceptualization, Methodology, Formal analysis, Investigation, Writing-original draft, Writing-review & editing. PV: Investigation, Formal analysis, Writing-review & editing. BM: Investigation. PG: Formal analysis. DMP: Investigation. DKJ: Writing-review & editing. NVV: Supervision, Project administration, Writing-review & editing. GD: Supervision, Project administration, Writing-review & editing. All authors contributed to the article and approved the submitted version. Funding Weight loss is one of the most important non-pharmacological interventions to lower BP. Numerous interrelated pathophysiologic mechanisms stimulate higher BP in obesity [ 12]. In overweight/obese individuals, accelerated vascular aging can lead to HTN due to inflammation, oxidative stress, and insulin resistance [ 13]. Obese individuals also experience increased activity of the sympathetic nervous system and the renin-angiotensin-aldosterone system [ 14]. The combined effect results in increased sodium resorption by the kidney, impaired vasodilation, volume expansion, and decreased natriuresis, thus leading to elevated BP [ 15]. Ozemek et al. trials have shown that weight loss helped lower systolic BP in hypertensive individuals by 5 mm Hg and in normotensive individuals by 2 to 3 mm Hg. According to the Centers for Disease Control (CDC), a BMI of 25 to 29.9 is considered overweight, and a BMI of 30 or higher is considered obese. In overweight or obese individuals, achieving their ideal body weight is best, but it is good to aim for at least a 1 kg reduction in body weight. The study by Ozemek et al. also revealed that for every 1 kg reduction in body weight, we can expect about a 1 mm Hg reduction in blood pressure.

Assorted studies have shown that IF lowers BP. Harvie et al., in their study involving 107 overweight or obese premenopausal women, showed that IF for six months helped lower SBP (p= 0.99) and DBP (p= 0.84) [ 22]. Varady et al., in their study performed for 12 weeks with IF involving 15 overweight individuals (five males, 10 females with a BMI of 20-29.9 kg/m 2 showed that IF helped lower BP with a p-value of 0.51 [ 23]. A study by Bhutani et al. involving 83 obese individuals (three males and 80 females) with a BMI of 30-39.9 kg/m 2revealed that 12 weeks of IF helped lower SBP (p = 0.254) and DBP (p = 0.570) [ 24]. In a study by Eshghinia et al., 15 overweight or obese women with a BMI ≥25 kg/m 2 who followed IF for eight weeks showed a lowering of SBP (p<0.001) and DBP (p<0.05) [ 25]. Teng et al., in their 12 weeks IF study with 28 Malay men with a BMI of 23-29.9 kg/m 2,showed a lowering in SBP (p<0.05) and DBP (p<0.05) [ 26]. Erdem et al., in their study involving 60 participants from the Cappadocia cohort with pre-HTN and HTN with SBP of 120-139 and more than or equal to 140 mm Hg, DBP of 80-80 and more than or equal to 90 mm Hg, revealed that IF helps lower SBP (p<0.001) and DBP (p<0.039) [ 27]. When the isolate NIOT-EQR_J7 was grown in the presence of Hg 2+ supplementation, a slight reduction in the peak intensity attributed to the thiol group (S-H stretch) at 2383.09 cm -1 was observed as compared to the control along with the wavelength that shifted to 2442.42 cm -1. In contrast, the peak intensity corresponding to the thiol group of NIOT-EQR_J248 at 2304.55 cm -1 increased compared to without Hg 2+ supplementation and the shift in wavelength to 2246.72 cm -1 was observed. In the case of NIOT-EQR_J251, the peaks corresponding to the S-H group appeared at 2341.34 cm -1 in the presence of Hg 2+ whereas, in the absence of Hg 2+, the peak was negligible. The peak was absent in both the presence and absence of Hg 2+ in the case of isolate NIOT-EQR_J258 ( Figures5A–D). In accord with our findings, previous studies had also reported similar results ( Dash and Das, 2014; De etal., 2014; Joshi etal., 2021) and suggested significant changes in the FT-IR spectrum such as a shift in wavenumber in the region of O-H groups, –S-H group, amide C=O, Nitrile C–N stretch, phosphate groups, etc. The changes in peak height and area have also been observed when isolates were grown in the presence of Hg 2+ ions compared to the controlled ones. The transmittance of the infrared (IR) in the presence of Hg 2+ was lower than in the absence of Hg 2+. The changes suggested that the presence of metal leads to a lesser degree of bond stretching, consequently reducing the IR transmittance. The contribution of functional groups in metal binding was legitimized undeniably by the formation of varying spectral bands with and without Hg ( Oves etal., 2013). Briasoulis et al., in their meta-analysis of 16 prospective long-term studies, revealed that alcohol consumption of more than 20 g per day increases the risk of HTN significantly in women, whereas consumption of 31 to 40 g in men significantly increases the risk of HTN [ 54]. Nevertheless, a higher risk of HTN was observed in all individuals who consumed more than 20 g of alcohol per day, irrespective of their gender [ 54]. Ozemek et al. revealed in their study that, in persons who consume alcohol, a reduction to two standard drinks or less daily in men and one drink or less daily in women has been shown to reduce SBP by 4 mm Hg in hypertensive individuals and by 3 mm Hg in normotensive individuals [ 11]. Spray the HG car dashboard cleaner thinly and evenly onto the surface to be treated. Keep the can upright and approximately 20cm away from the dashboard. A simplified X-ray film method was adopted to look into the Hg reduction by bacterial volatilization in the presence of 50 and 100 mg/L of Hg as HgCl 2 ( Nakamura and Nakahara, 1988; Joshi etal., 2021). Briefly, the bacterial cells were collected by centrifugation at 5,000 rpm for 10 min. and washed with 0.07 M phosphate buffer (0.5 mM EDTA, 0.2 mM magnesium acetate, 5 mM sodium thioglycolate; pH 7.0) and transferred to the microplate. The cells were suspended into 50 µL of 0.07 M phosphate buffer containing 50 and 100 mg/L of Hg as HgCl 2 in a microplate. The phosphate buffers (pH 7.0) with 50 and 100 mg/L of HgCl 2 (without bacterial cells) were used as a negative control. The plate was covered with X-ray film and incubated in the dark for 2 h at 35°C. Determination of Hg(II) depletion potential by isolates